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	<title>Comments on: PMSF</title>
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	<link>http://protocolsonline.com/recipes/pmsf-10-mm-10-ml/</link>
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	<lastBuildDate>Fri, 02 Mar 2012 08:25:15 +0000</lastBuildDate>
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	<item>
		<title>By: Harekrushna Panda</title>
		<link>http://protocolsonline.com/recipes/pmsf-10-mm-10-ml/comment-page-1/#comment-285</link>
		<dc:creator>Harekrushna Panda</dc:creator>
		<pubDate>Mon, 06 Feb 2012 18:39:20 +0000</pubDate>
		<guid isPermaLink="false">http://protocolsonline.com/?p=450#comment-285</guid>
		<description>CORRECT RECIPE FOR PREPARATION OF 10mM PMSF (10 ml) 
Preparation of PMSF (10 mM), 10 ml

    Weigh out  17.4mg PMSF
    Add isopropanol to 10ml and dissolve.
    No need to filter sterilize.
    Aliquot and store at –20°C.</description>
		<content:encoded><![CDATA[<p>CORRECT RECIPE FOR PREPARATION OF 10mM PMSF (10 ml)<br />
Preparation of PMSF (10 mM), 10 ml</p>
<p>    Weigh out  17.4mg PMSF<br />
    Add isopropanol to 10ml and dissolve.<br />
    No need to filter sterilize.<br />
    Aliquot and store at –20°C.</p>
]]></content:encoded>
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	<item>
		<title>By: jxe09</title>
		<link>http://protocolsonline.com/recipes/pmsf-10-mm-10-ml/comment-page-1/#comment-151</link>
		<dc:creator>jxe09</dc:creator>
		<pubDate>Sun, 20 Mar 2011 06:32:29 +0000</pubDate>
		<guid isPermaLink="false">http://protocolsonline.com/?p=450#comment-151</guid>
		<description>Thanks for pointing out the typo; this has been updated.</description>
		<content:encoded><![CDATA[<p>Thanks for pointing out the typo; this has been updated.</p>
]]></content:encoded>
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	<item>
		<title>By: biowow</title>
		<link>http://protocolsonline.com/recipes/pmsf-10-mm-10-ml/comment-page-1/#comment-150</link>
		<dc:creator>biowow</dc:creator>
		<pubDate>Tue, 01 Mar 2011 23:15:11 +0000</pubDate>
		<guid isPermaLink="false">http://protocolsonline.com/?p=450#comment-150</guid>
		<description>This is not correct, go back to school, PMSF MW is 174.2... -&gt; 10mM, 10ml =0.017g</description>
		<content:encoded><![CDATA[<p>This is not correct, go back to school, PMSF MW is 174.2&#8230; -&gt; 10mM, 10ml =0.017g</p>
]]></content:encoded>
	</item>
	<item>
		<title>By: Nicole</title>
		<link>http://protocolsonline.com/recipes/pmsf-10-mm-10-ml/comment-page-1/#comment-146</link>
		<dc:creator>Nicole</dc:creator>
		<pubDate>Fri, 10 Dec 2010 13:44:25 +0000</pubDate>
		<guid isPermaLink="false">http://protocolsonline.com/?p=450#comment-146</guid>
		<description>Are you sure &quot;g&quot; not &quot;mg&quot;? :)</description>
		<content:encoded><![CDATA[<p>Are you sure &#8220;g&#8221; not &#8220;mg&#8221;? <img src='http://protocolsonline.com/wp-includes/images/smilies/icon_smile.gif' alt=':)' class='wp-smiley' /> </p>
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