Paraffin processing of tissue

Paraffin processing of tissue

Learn about the technique of paraffin embedding, a classic method used in histology.

Haematoxylin Eosin (H&E) staining

Haematoxylin Eosin (H&E) staining

H&E stains are the most common staining technique used for histologic studies.

Weil’s myelin stain

Weil’s myelin stain

Weil's stain is a simple technique that is used to stain myelin in the nervous system, involving the reduction of chrome salt to chromium dioxide by myelin.

Pipette

Pipette

Find out here about the common laboratory pipette, how it works, and how to use one effectively!

c-Fos

c-Fos

Learn how to stain tissue for c-Fos, a cellular proto-oncogene and indirect indicator of neural activity.

Phosphate buffered saline

Phosphate buffered saline

Read about the preparation of phosphate buffered saline, a solution that is commonly used in biological research.

Mordant

Mordant

11 April 2010

Find out about mordants, substance used to set dyes on tissue sections.

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PMSF

PMSF

11 April 2010

PMSF is commonly used in protein solublization in order to deactivate proteases from digesting proteins of interest after cell lysis.

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Ampicillin

Ampicillin

11 April 2010

Ampicillin is a beta-lactam antibiotic used as a selective agent in molecular biology to select for the uptake of genes by bacteria.

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Acrylamide

Acrylamide

11 April 2010

Acrylamide solution is used for the creation of polyacrylamide gels in gel electrophoresis techniques, such as western blotting.

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Two-dimensional gel electrophoresis

Two-dimensional gel electrophoresis

11 April 2010

Learn about two-dimensional gel electrophoresis, a form of gel electrophoresis commonly used to analyze multiple characteristics of proteins.

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A gentle introduction to western blotting

A gentle introduction to western blotting

11 April 2010

Learn about western blotting, an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract.

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